SNX1 Antibody

Western Blot: SNX1 Antibody [NBP1-46150] - Staining of HeLa cell lysates with antibody at 1 ug/mL (A) and U2OS (B) cell lysates with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: SNX1 Antibody [NBP1-46150] - Staining of formaldehyde-fixed SH5Y5Y with antibody at 5 ug/mL and detected with Alexa 488 in confocal microscopy. Data obtained from Dr. Schallburg Nielsen, Aarhus University Denmark.

Immunohistochemistry-Paraffin: SNX1 Antibody [NBP1-46150] - Staining of paraffin embedded Human Uterus with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SNX1 Antibody Summary

• Immunogen: Peptide with sequence C-TNSSKPQPTYEELE corresponding to internal region according to NP_690039.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 3 - 5 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX1 Antibody

• HsT17379
• MGC8664
• SNX1Asorting nexin 1A
• sorting nexin 1
• sorting nexin-1
• Vps5

Background

Sorting nexin 1 (SNX1) is a component of the retromer complex that functions in endosome to trans-golgi network protein trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX1 Antibody (NBP1-46150)(1)

Publication using NBP1-46150

• Mari M, Bujny MV, Zeuschner D et al. SNX1 defines an early endosomal recycling exit for sortilin and mannose 6-phosphate receptors. Traffic 2008-03-01 [PMID: 18088323]

Bioinformatics

• Gene Symbol: SNX1
• Entrez:
  • 6642(Human)
  • 56440(Mouse)
  • 84471(Rat)
• Uniprot:
  • NP_003090.2(Human)