SNX1 Antibody (JG40-06)

Western Blot: SNX1 Antibody (JG40-06) [NBP2-75670] - Analysis of SNX1 on human skin tissue lysate using anti-SNX1 antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: SNX1 Antibody (JG40-06) [NBP2-75670] - Staining SNX1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SNX1 Antibody (JG40-06) [NBP2-75670] - Analysis of paraffin-embedded rat epididymis tissue using anti-SNX1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SNX1 Antibody (JG40-06) [NBP2-75670] - Analysis of SiHa cells with SNX1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: SNX1 Antibody (JG40-06) [NBP2-75670] - Staining SNX1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SNX1 Antibody (JG40-06) [NBP2-75670] - Staining SNX1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SNX1 Antibody (JG40-06) [NBP2-75670] - Analysis of paraffin-embedded human colon tissue using anti-SNX1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SNX1 Antibody (JG40-06) [NBP2-75670] - Analysis of paraffin-embedded human breast cancer tissue using anti-SNX1 antibody. Counter stained with hematoxylin.

Product Discontinued

SNX1 Antibody (JG40-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SNX1 aa 1-140 / 522. (SwissProt: Q13596 Human; SwissProt: Q99N27 Rat)
• Localization: Endosome. Golgi apparatus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SNX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SNX1 Antibody (JG40-06)

• HsT17379
• MGC8664
• SNX1Asorting nexin 1A
• sorting nexin 1
• sorting nexin-1
• Vps5

Background

SNX1 encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNX1