SNAP alpha/beta Antibody (6B7-3)

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Immunohistochemistry: SNAP alpha/beta Antibody (6B7-3) [NBP1-19335] - Immunohistochemistry analysis of human brain, cortex tissue stained with SNAP a/beta, mAb (6B7-3) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
6B7-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.33 mg/ml

Order Details

SNAP alpha/beta Antibody (6B7-3) Summary

Immunogen
Recombinant bovine alpha-SNAP protein
Specificity
SNAP alpha/beta (6B7-3)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NAPA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Human, Mouse, Rat, Bovine and Canine. May react with Hamster and Sheep. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
Sodium Azide
Concentration
0.33 mg/ml
Purity
Protein G purified

Alternate Names for SNAP alpha/beta Antibody (6B7-3)

  • alpha-SNAP
  • alpha-soluble NSF attachment protein
  • N-ethylmaleimide-sensitive factor attachment protein alpha
  • N-ethylmaleimide-sensitive factor attachment protein, alpha
  • S
  • SNAPA
  • SNAP-alpha

Background

Alpha, beta and gamma SNAPs, also known as soluble NSF attachment proteins, have apparent molecular weights on SDS PAGE of 35, 36 and 39 kDa, respectively. SNAP proteins mediate the association of NSF to membranes during vesicle fusion reaction, and play a prominent role in synaptic vesicle fusion. In addition, beta SNAP interacts with synaptotagmin and may be involved in calcium regulated exocytosis. SNAP was identified as being required for vesicle fusion in intra Golgi transport reaction in vitro. Alpha SNAP mediates the association of NSF to membranes during vesicle fusion reaction, and plays a prominent role and in synaptic vesicle fusion. Together with synaptotagmin and NSF, alpha SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in, an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NAPA
Entrez