SMARCA5/SNF2H Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

SMARCA5/SNF2H Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human SAMRCA5/SNF2H
  Asp77-Val185
  Accession # O60264
• Specificity: Detects human SMARCA5/SNF2H in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SMARCA1 and rhSMARC4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMARCA5/SNF2H Antibody [Alexa Fluor® 647]

• EC 3.6.1
• EC 3.6.4.-
• hISWI
• hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
• ISWI
• SMARCA5
• SNF2H
• SNF2Hsucrose nonfermenting-like 5
• subfamily a, member 5
• Sucrose nonfermenting protein 2 homolog
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5
• WCRF135

Background

SMARCA5 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 5; also SNF2H) is a 130-135 kDa member of the SNF2/RAD54 helicase family of molecules. It is a ubiquitously expressed, chromatin remodeling ATPase that increases the accessibility of nucleosomal DNA to DNA-binding proteins. It apparently does so by sliding histone octamers over DNA. SMARCA5 is a component of four chromatin-remodeling complexes, including CHRAC, RSF, ACF and WICH. Human SMARCA5 is 1052 amino acids (aa) in length. It contains an N-terminal helicase domain (aa 192-357), a helicase superfamily C-terminal domain (aa 474-605), and two SANT DNA-binding domains (aa 840-892 and 943-1007). Over aa 77-185, human SMARCA5 shares 99% aa identity with mouse SMARCA5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.