SMAP2 Antibody

Images

 
Western Blot: SMAP2 Antibody [NBP1-80737] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: SMAP2 Antibody [NBP1-80737] - Staining of human lymph node shows strong positivity in cells outside the germinal center.
Orthogonal Strategies: Western Blot: SMAP2 Antibody [NBP1-80737] - Analysis in human cell lines PC-3 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SMAP2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
SMAP2 Protein (NBP1-80737PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SMAP2 Antibody

  • RP1-228H13.3
  • small ArfGAP2
  • SMAP1L
  • SMOC2
  • stromal membrane-associated GTPase-activating protein 2
  • Stromal membrane-associated protein 1-like
  • stromal membrane-associated protein 2

Background

GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role inclathrin-dependent retrograde transport from early endosomes to the trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-80737

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Bioinformatics

Gene Symbol SMAP2
Entrez