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SLC39A7/ZIP7 Overexpression Lysate

Images

 
Western Blot: SLC39A7/ZIP7 Overexpression Lysate (Adult Normal) [NBL1-16150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC39A7/ZIP7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SLC39A7/ZIP7 Overexpression Lysate Summary

Description
Transient overexpression lysate of solute carrier family 39 (zinc transporter), member 7 (SLC39A7), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC200556 and based on accession number NM_006979. The protein contains a C-MYC/DDK Tag.
Gene
SLC39A7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
49.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC39A7/ZIP7 Overexpression Lysate

  • D6S115E
  • D6S2244EReally interesting new gene 5 protein
  • H2-KE4
  • HKE4Solute carrier family 39 member 7
  • HLA class II region expressed gene KE4
  • Ke4 gene, mouse, human homolog of
  • KE4
  • RING5Histidine-rich membrane protein Ke4
  • solute carrier family 39 (zinc transporter), member 7
  • zinc transporter SLC39A7
  • ZIP7

Background

Zinc is an essential cofactor for more than 50 classes of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. Zinc cannot passively diffuse across cell membranes and requires specific transporters, such as SLC39A7, to enter the cytosol from both the extracellular environment and from intracellular storage compartments.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SLC39A7