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SLC35A3 Antibody [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-98629AF350
Alexa Fluor 405
NBP2-98629AF405
Alexa Fluor 488
NBP2-98629AF488
Alexa Fluor 532
NBP2-98629AF532
Alexa Fluor 594
NBP2-98629AF594
Alexa Fluor 647
NBP2-98629AF647
Alexa Fluor 700
NBP2-98629AF700
Alexa Fluor 750
NBP2-98629AF750
Biotin
NBP2-98629B
DyLight 350
NBP2-98629UV
DyLight 405
NBP2-98629V
DyLight 488
NBP2-98629G
DyLight 550
NBP2-98629R
DyLight 594
NBP2-98629DL594
DyLight 680
NBP2-98629FR
DyLight 755
NBP2-98629IR
FITC
NBP2-98629F
HRP
NBP2-98629H
Janelia Fluor 549
NBP2-98629JF549
Janelia Fluor 646
NBP2-98629JF646
Unconjugated
NBP2-98629-100ul
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SLC35A3 Antibody [DyLight 650] Summary

Immunogen
Produced in rabbits immunized with E. coli-derived Human SLC35A3 fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC35A3
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for SLC35A3 Antibody [DyLight 650]

  • DKFZp781P1297
  • Golgi UDP-GlcNAc transporter
  • member 3
  • member A3
  • solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter)
  • Solute carrier family 35 member A3
  • UDP-N-acetylglucosamine transporter

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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UGT Antibody
NBP1-80642
Immunocytochemistry/Immunofluorescence: UGT Antibody [NBP1-80642] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UGT Antibody [NBP1-80642] - Staining of human rectum shows strong cytoplasmic staining and additional nuclear membrane positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Recombinant Human
SLC35A1 GST (N-Term) ...
H00010559-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
UGT1A9 Antibody
H00054600-B01P
Western Blot: UGT1A9 Antibody [H00054600-B01P] - Analysis of UGT1A9 expression in human liver.
Immunohistochemistry-Paraffin: UGT1A9 Antibody [H00054600-B01P] - Analysis of purified antibody to UGT1A9 on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)
Western Blot: UGT1A9 Antibody [H00054600-B01P] - Analysis of UGT1A9 expression in transfected 293T cell line by UGT1A9 polyclonal antibody. Lane 1: UGT1A9 transfected lysate(58.3 KDa). Lane 2: Non-transfected lysate.
(3 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P
UGT1A6 Antibody
NBP1-33743
Western Blot: UGT1A6 Antibody [NBP1-33743] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UGT1A6 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: UGT1A6 Antibody [NBP1-33743] - Paraffin-embedded OV90 xenograft, using antibody at 1:100 dilution.
Western Blot: UGT1A6 Antibody [NBP1-33743] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Recombinant Human
UGT1A10 GST (N-Term) ...
H00054575-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu

Glucuronosyltransferas ...
AF6490
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Glucuronosyltransferase  1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6490) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent‑Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6490) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu
Applications: WB, IHC, ICC
UGT1A3 Antibody
(1C10)
H00054659-M02
ELISA: UGT1A3 Antibody (1C10) [H00054659-M02] - Detection limit for recombinant GST tagged UGT1A3 is 1 ng/ml as a capture antibody.
(3 Publications)
Species: Hu
Applications: WB, ELISA, S-ELISA
UGT1A4 Antibody
NBP2-94258
Western Blot: UGT1A4 Antibody [NBP2-94258] - Analysis of extracts of various cell lines, using UGT1A4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry-Paraffin: UGT1A4 Antibody [NBP2-94258] - Paraffin-embedded human kidney cancer using UGT1A4.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
UGCGL2 Antibody
NBP2-20803
Western Blot: UGCGL2 Antibody [NBP2-20803] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: UGCGL2 Antibody [NBP2-20803] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: UGCGL2 protein stained by UGCGL2 antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: UGCGL2 Antibody [NBP2-20803] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
RAB14 Antibody
NBP1-84720
Western Blot: RAB14 Antibody [NBP1-84720] - Analysis in human cell line RH-30.
Immunocytochemistry/Immunofluorescence: RAB14 Antibody [NBP1-84720] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ITM2A Antibody
AF4876
Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITM2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4876) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ITM2A (glycosylated) at approximately 45 kDa and ITM2A (not glycosylated) at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB
SLC4A4 Antibody
NBP2-94138
Western Blot: SLC4A4 Antibody [NBP2-94138] - Analysis of extracts of 293T cells, using SLC4A4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time:
Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-94138] - Paraffin-embedded rat kidney using SLC4A4.
Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-94138] - Paraffin-embedded human prostate using SLC4A4.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Twist-1 Antibody
(10E4E6)
NBP2-37364
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
(4 Publications)
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC, KD
SLC35B4 Antibody
NBP2-13329
Immunohistochemistry-Paraffin: SLC35B4 Antibody [NBP2-13329] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
SLC13A2 Antibody
NBP1-82604
Immunohistochemistry-Paraffin: SLC13A2 Antibody [NBP1-82604] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: SLC13A2 Antibody [NBP1-82604] - Staining of human small intestine shows strong luminal membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: SLC13A2 Antibody [NBP1-82604] - Staining of human duodenum shows high expression.
Species: Hu
Applications: IHC, IHC-P
CD117/c-kit
Antibody [Unconjugate
AF1356
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(4 Reviews)
(29 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
Glycophorin C
Antibody
NBP1-84597
Western Blot: Glycophorin C Antibody [NBP1-84597] - Analysis in control (vector only transfected HEK293T lysate) and GYPC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP1-84597] - Staining in human bone marrow and pancreas tissues using anti-GYPC antibody. Corresponding GYPC RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP1-84597] - Staining of human colon shows strong immunoreactivity in erythrocytes present in blood vessel and moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
SLC35A3 Antibody
[DyLight 650]
NBP2-98629C
SLC35A3 Antibody [DyLight 650]
Species: Hu
Applications: IHC, IHC-P

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-98629AF350
Alexa Fluor 405 NBP2-98629AF405
Alexa Fluor 488 NBP2-98629AF488
Alexa Fluor 532 NBP2-98629AF532
Alexa Fluor 594 NBP2-98629AF594
Alexa Fluor 647 NBP2-98629AF647
Alexa Fluor 700 NBP2-98629AF700
Alexa Fluor 750 NBP2-98629AF750
Biotin NBP2-98629B
DyLight 350 NBP2-98629UV
DyLight 405 NBP2-98629V
DyLight 488 NBP2-98629G
DyLight 550 NBP2-98629R
DyLight 594 NBP2-98629DL594
DyLight 650 NBP2-98629C
DyLight 680 NBP2-98629FR
DyLight 755 NBP2-98629IR
FITC NBP2-98629F
HRP NBP2-98629H
Janelia Fluor 549 NBP2-98629JF549
Janelia Fluor 646 NBP2-98629JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol SLC35A3