SLC26A7 Antibody (14H5)

Western Blot: SLC26A7 Antibody (14H5) [NB110-60965] - Detection of Slc26a7 using NB110-60965. Tx: Okp cells transiently transfected with mouse Slc26a7 UTX: untransfected Okp cells.

Immunocytochemistry/Immunofluorescence: SLC26A7 Antibody (14H5) [NB110-60965] - SLC26A7 antibody was tested in HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

Product Discontinued

SLC26A7 Antibody (14H5) Summary

• Immunogen: A fusion protein containing the C-terminal region of mouse SLC26A7. [Swiss-Prot# Q24JS8]
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC26A7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: This SLC26A7 antibody is useful for Western blot on cells transiently transfected with mouse SLC26A7 and also for Immunocytochemistry/Immunofluorescence. In WB a band can be seen at ~90-100 kDa representing SLC26A7. Note: Do not boil the samples.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33583874). Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.7 mg/ml
• Purity: Protein G purified

Alternate Names for SLC26A7 Antibody (14H5)

• MGC126268
• solute carrier family 26, member 7
• sulfate anion transporter

Background

SLC26A7 (solute carrier family 26 member 7) is a chloride-base exchanger and/or chloride transporter that is expressed on basolateral membrane of acid-secreting cells in renal outer medullary collecting duct (OMCD) and in gastric parietal cells also. SLC26A7 belongs to conserved family of anion transporters SLC26 isoforms which display tissue-specific expression patterns in epithelial cells and several SLC26 members function as chloride/bicarbonate exchangers that include SLC26A3 (DRA), SLC26A4 (pendrin), SLC26A6 (PAT1 or CFEX), SLC26A7, and SLC26A9 among which SLC26A7 and SLC26A9 also function as chloride channels. In renal tissues, Slc26a7 co-localizes with AE1, a well-known Cl-/HCO3- exchanger, on the basolateral membrane of (acid-secreting) A-intercalated cells in OMCD cells, and in the gastric tissue, SLC26A7 co-localizes with AE2, a major Cl-/HCO3- exchanger, on the basolateral membrane of acid secreting parietal cells. SLC26A7 acts as a sodium-independent DIDS-sensitive anion exchanger mediating bicarbonate, chloride, sulfate and oxalate transport. SLC26A7 maintains the electrolyte/acid-base homeostasis in kidneys by acting as a distal excretory segment-specific anion exchanger, specifically chloride, and plays a major role in gastric acid secretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC26A7 Antibody (NB110-60965)(2)

Publications using NB110-60965

• Tanimura Y, Kiriya M, Kawashima A et al. Regulation of solute carrier family 26 member 7 (Slc26a7) by thyroid stimulating hormone in thyrocytes Endocrine journal 2021-02-14 [PMID: 33583874] (WB, Rat)
• Dudas, PL et al. Immunolocalization of anion transporter Slc26a7 in mouse kidney. Am J Physiol Renal Physiol;290(4):F937-45. 2006-04-01 [PMID: 16263805] (ICC/IF, WB, Mouse)

Bioinformatics

• Gene Symbol: SLC26A7
• Entrez:
  • 115111(Human)
  • 208890(Mouse)
• Uniprot:
  • Q8R2Z3(Mouse)