SLC25A25 Knockout HeLa Cell Lysate

Product Discontinued

SLC25A25 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-2 bp deletion in exon1 and 1 bp insertion in exon1
• Gene: SLC25A25

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SLC25A25 Knockout HeLa Cell Lysate

• APC3
• calcium-binding mitochondrial carrier protein SCaMC-2
• KIAA1896RP11-395P17.4
• MCSC
• MCSC3
• MGC105138
• MGC119514
• MGC119515
• MGC119516
• MGC119517
• Mitochondrial ATP-Mg/Pi carrier protein 3
• Mitochondrial Ca(2+)-dependent solute carrier protein 3
• mitochondrial Ca2+-dependent solute carrier
• PCSCL
• SCAMC2
• SCAMC-2
• short calcium-binding mitochondrial carrier 2
• small calcium-binding mitochondrial carrier 2
• Small calcium-binding mitochondrial carrier protein 2
• solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
• Solute carrier family 25 member 25
• solute carrier family 25, member 25

Background

The protein encoded by this gene belongs to the family of calcium-binding mitochondrial carriers, with a characteristic mitochondrial carrier domain at the C-terminus. These proteins are found in the inner membranes of mitochondria, and function as transport proteins. They shuttle metabolites, nucleotides and cofactors through the mitochondrial membrane and thereby connect and/or regulate cytoplasm and matrix functions. This protein may function as an ATP-Mg/Pi carrier that mediates the transport of Mg-ATP in exchange for phosphate, and likely responsible for the net uptake or efflux of adenine nucleotides into or from the mitochondria. Alternatively spliced transcript variants encoding different isoforms with a common C-terminus but variable N-termini have been described for this gene. [provided by RefSeq, Jul 2012]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SLC25A25