SLC19A3 Antibody

Images

 
Western Blot: SLC19A3 Antibody [NBP1-86943] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed ...read more
Immunocytochemistry/ Immunofluorescence: SLC19A3 Antibody [NBP1-86943] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SLC19A3 Antibody [NBP1-86943] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SLC19A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:KKSSSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECY
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC19A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:250
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SLC19A3 Protein (NBP1-86943PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-86943 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC19A3 Antibody

  • Solute carrier family 19 member 3
  • solute carrier family 19, member 3
  • thiamine transporter 2
  • THTR2
  • ThTr-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC19A3 Antibody (NBP1-86943) (0)

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Review for SLC19A3 Antibody (NBP1-86943) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-86943:
Filter by Applications
IF
(1)
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Human
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Images Ratings Applications Species Date Details
Immunofluorescence SLC19A3 NBP1-86943
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reviewed by:
Anonymous
IF Human 03/28/2014
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Summary

ApplicationImmunofluorescence
Sample TestedCultured Saos2 Cells
SpeciesHuman
Lotab135046
Commentsantibody works well, slightly high background

Blocking

Blocking DetailsTBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:5000 in TBST, 1 hour at room temperature

Secondary Antibody

Secondary Descriptionanti-rabbit Alexa594
Secondary Manufacturer Cat#ab150092
Secondary Concentration1:1000 in TBST

Details

Detection NotesOpera high-throughput confocal microscope
Fixation DetailsFixed for 20 minutes with paraformaldehyde, permeabilized with TBST
Wash DescriptionTBST, 3 washes at 5 minutes each

Comments

Commentsantibody works well, slightly high background

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC19A3 Antibody (NBP1-86943) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SLC19A3 Antibody (NBP1-86943)

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Recent Reviews

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Anonymous
03/28/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol SLC19A3