SIGLECL12 Antibody

Product Discontinued

SIGLECL12 Antibody Summary

• Immunogen: Peptide with sequence C-EAIGYEYSEINIPK corresponding to C-Terminus according to NP_201586.1, NP_443729.1.
• Localization: Cell Membrane
• Specificity: This antibody is expected to recognise both human isoforms of this protein (NP_201586.1; NP_443729.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave no signal but low background in human PBMC and Molt-4 lysates at up to 1 ug/ml.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for SIGLECL12 Antibody

• FLJ38600
• S2V
• sialic acid binding Ig-like lectin 12
• sialic acid-binding Ig-like lectin 12
• Siglec-12
• SIGLECL1
• SIGLECL1Sialic acid-binding Ig-like lectin-like 1
• SIGLEC-like 1
• Siglec-XII
• SLG
• SLGSiglec-L1

Background

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to theimmunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialicacid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily ofSIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed bytwo C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encodedprotein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containingprotein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negativeregulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster withother SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have beendescribed for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIGLECL12 Antibody (NB100-1174)(1)

Publication using NB100-1174

• Yu Z, Lai CM, Maoui M et al. Identification and characterization of S2V, a novel putative siglec that contains two V set Ig-like domains and recruits protein-tyrosine phosphatases SHPs. J Biol Chem 2001-06-29 [PMID: 11328818]

Bioinformatics

• Gene Symbol: SIGLEC12
• Entrez:
  • 89858(Human)
• Uniprot:
  • NP_201586(Human)