Shrew-1/AJAP1 Antibody

Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (thalamus/hypothalamus) tissue, and mouse pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Shrew-1/AJAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7970) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shrew-1/ AJAP1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Shrew-1/AJAP1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Shrew-1/AJAP1
  His157-His283
  Accession # A2ALI5
• Specificity: Detects mouse Shrew-1/AJAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human AJAP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AJAP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Shrew-1/AJAP1 Antibody

• adherens junction associated protein 1
• adherens junction-associated protein 1
• adherens junctions associated protein 1
• AJAP1
• Gm573
• membrane protein shrew-1
• MOT8
• RP3-426F10.1
• Shrew1
• Shrew-1
• transmembrane protein SHREW1

Background

AJAP-1 (Adherens Junction Associated Protein 1; also Shrew-1 and Gm573) is a 44-47 kDa transmembrane protein found on the basolateral surface of epithelium. It is presumably expressed by multiple types of epithelium, and participates in cell-cell adhesion. Zonula adherens are structures that participate in the generation of cell contact adherens junctions. Such junctions, or complexes, are key to the maintenance of epithelial cell polarity and the maintenance of cell-to-cell contact. Central to this complex is E-cadherin, a transmembrane molecule that makes crucial contacts with cytoskeletal actin via catenins. AJAP-1 is an accompaning transmembrane molecule that interacts with E-cadherin and appears to promote the internalization of E-cadherin upon growth factor stimulation. This facilitates the dissolution of adherens junctions with an increase in cell motility. Notably, AJAP-1 is also known to complex with CD147 in nonpolar, invasive cells, suggesting that AJAP-1 may play a more complex role in cell migration. Mouse AJAP-1 is a 412 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 284 aa extracellular region (aa 1-284) plus a 106 aa cytoplasmic domain (aa 306-412). Over aa 1-283, mouse AJAP-1 shares 95% and 75% aa sequence identity with rat and human AJAP-1, respectively; over aa 158-283, mouse AJAP-1 sequence identity changes little, showing 95% and 78% equality with rat and human AJAP-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Shrew-1/AJAP1 Antibody (AF7970)(4)

Publications using AF7970

• Früh, S;Boudkkazi, S;Koppensteiner, P;Sereikaite, V;Chen, LY;Fernandez-Fernandez, D;Rem, PD;Ulrich, D;Schwenk, J;Chen, Z;Le Monnier, E;Fritzius, T;Innocenti, SM;Besseyrias, V;Trovò, L;Stawarski, M;Argilli, E;Sherr, EH;van Bon, B;Kamsteeg, EJ;Iascone, M;Pilotta, A;Cutrì, MR;Azamian, MS;Hernández-García, A;Lalani, SR;Rosenfeld, JA;Zhao, X;Vogel, TP;Ona, H;Scott, DA;Scheiffele, P;Strømgaard, K;Tafti, M;Gassmann, M;Fakler, B;Shigemoto, R;Bettler, B; Monoallelic de novo AJAP1 loss-of-function variants disrupt trans-synaptic control of neurotransmitter release Science advances 2024-07-12 [PMID: 38985877] (Immunohistochemistry, Immunoprecipitation, Human, Mouse)
• MC Dinamarca, A Raveh, A Schneider, T Fritzius, S Früh, PD Rem, M Stawarski, T Lalanne, R Turecek, M Choo, V Besseyrias, W Bildl, D Bentrop, M Staufenbie, M Gassmann, B Fakler, J Schwenk, B Bettler Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing Nat Commun, 2019-03-22;10(1):1331. 2019-03-22 [PMID: 30902970] (Binding Assay, Immunoprecipitation, Western Blot, Human, Mouse)
• Anna Starzinski Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile Biol Open, 2016-11-15;5(11):1607-1619. 2016-11-15 [PMID: 27870635] (IHC-P, Immunoprecipitation, Mouse)
• Schwenk J, Perez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca M, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics. Nat Neurosci, 2015-12-21;19(2):233-42. 2015-12-21 [PMID: 26691831] (IHC-Fr, Mouse, Rat)

Bioinformatics

• Gene Symbol: AJAP1
• Uniprot:
  • A2ALI5()