SHIP2/INPPL1 Antibody (737935) [Alexa Fluor® 594]

• Applications: WB, ICC/IF
• Clone: 737935
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

SHIP2/INPPL1 Antibody (737935) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human SHIP2
  Ala1106-Lys1258
  Accession # O15357
• Specificity: Detects human SHIP2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse SHIP2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

SHIP2 (SH2 domain-containing inositol 5-phosphatase 2; also inositol polyphosphate phosphatase-like 1) is a 160 kDa member of the phosphatidylinositol/PtdIns 5-phosphatase family of enzymes.  It is widely expressed and negatively regulates PI3 kinase pathways by hydrolyzing the 5-phosphate of PtdIns-3,4,5-triphosphate.  Human SHIP2 is 1258 amino acids (aa) in length.  It contains one SH2 domain (aa 21-117), a Pro-rich region with an SH3 binding site (aa 935-1105), and a SAM (or sterile alpha -motif) domain that binds ARAP3 (aa 1196-1258). Phosphorylation is known to occur on Tyr986, 1162 and 1358, and on Thr958. There is an alternate start site at Met243 and a second splice variant that shows a 40 aa substitution for aa 1228-1258. Over aa 1106-1258, human SHIP2 shares 95% aa identity with mouse SHIP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.