SHIP2/INPPL1 Antibody (737935) [Alexa Fluor® 750]

• Applications: WB, ICC/IF
• Clone: 737935
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

SHIP2/INPPL1 Antibody (737935) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human SHIP2
  Ala1106-Lys1258
  Accession # O15357
• Specificity: Detects human SHIP2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse SHIP2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHIP2/INPPL1 Antibody (737935) [Alexa Fluor® 750]

• EC 3.1.3
• EC 3.1.3.n1
• inositol polyphosphate phosphatase-like 1
• Inositol polyphosphate phosphatase-like protein 1
• INPPL1
• INPPL-1
• phosphatidylinositol-34,5-trisphosphate 5-phosphatase 2
• Protein 51C
• SH2 domain-containing inositol phosphatase 2
• SH2 domain-containing inositol-5'-phosphatase 2
• SHIP2
• SHIP-2
• SHIP251C protein

Background

SHIP2 (SH2 domain-containing inositol 5-phosphatase 2; also inositol polyphosphate phosphatase-like 1) is a 160 kDa member of the phosphatidylinositol/PtdIns 5-phosphatase family of enzymes.  It is widely expressed and negatively regulates PI3 kinase pathways by hydrolyzing the 5-phosphate of PtdIns-3,4,5-triphosphate.  Human SHIP2 is 1258 amino acids (aa) in length.  It contains one SH2 domain (aa 21-117), a Pro-rich region with an SH3 binding site (aa 935-1105), and a SAM (or sterile alpha -motif) domain that binds ARAP3 (aa 1196-1258). Phosphorylation is known to occur on Tyr986, 1162 and 1358, and on Thr958. There is an alternate start site at Met243 and a second splice variant that shows a 40 aa substitution for aa 1228-1258. Over aa 1106-1258, human SHIP2 shares 95% aa identity with mouse SHIP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O15357()