SHIP Antibody (SY11-08)

Western Blot: SHIP Antibody (SY11-08) [NBP2-67387] - Western blot analysis of SHIP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: SHIP Antibody (SY11-08) [NBP2-67387] - Analysis of paraffin-embedded human tonsil tissue using anti-SHIP antibody. Counter stained with hematoxylin.

Flow Cytometry: SHIP Antibody (SY11-08) [NBP2-67387] - Analysis of Raji cells with SHIP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Western Blot: SHIP Antibody (SY11-08) [NBP2-67387] - Analysis of SHIP on different lysates using anti-SHIP antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: THP-1

Immunohistochemistry-Paraffin: SHIP Antibody (SY11-08) [NBP2-67387] - Analysis of paraffin-embedded human spleen tissue using anti-SHIP antibody. Counter stained with hematoxylin.

Product Discontinued

SHIP Antibody (SY11-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SHIP aa 1,064-1,189 / 1,189. (SwissProt: Q92835 Human)
• Localization: Cytoskeleton, Cell membrane, Cytoplasm, Membrane raft, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INPP5D
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SHIP Antibody (SY11-08)

• 145kD
• EC 3.1.3
• hp51CNMGC142142
• Inositol polyphosphate-5-phosphatase of 145 kDa
• inositol polyphosphate-5-phosphatase, 145kDa
• INPP5D
• MGC104855
• SHIP
• SHIPMGC142140

Background

SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3, 4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP1 to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INPP5D