Recombinant Human SHC1 His Protein

SDS-Page: Recombinant Human SHC1 Protein [NBP1-72374] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human SHC1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-474 of Human SHC1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV GGDPEVRKQM PPPPPCPAGR ELFDDPSYVN VQNLDKARQA VGGAGPPNPA INGSAPRDLF DMKPFEDALR VPPPPQSVSM AEQLRGEPWF HGKLSRREAE ALLQLNGDFL VRESTTTPGQ YVLTGLQSGQ PKHLLLVDPE GVVRTKDHRF ESVSHLISYH MDNHLPIISA GSELCLQQPV ERKL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SHC1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 53.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 200 mM NaCl, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SHC1 His Protein

• FLJ26504
• p66
• p66SHC
• SH2 domain protein C1
• SHC (Src homology 2 domain containing) transforming protein 1
• SHC (Src homology 2 domain-containing) transforming protein 1
• SHC
• SHC1
• SHCA
• SHC-transforming protein 1
• SHC-transforming protein 3
• SHC-transforming protein A
• Src homology 2 domain-containing-transforming protein C1

Background

SHC1 (SHC-transforming protein 1) belongs to the SH2 domain family. SHC1 has been found to be important in the regulation of apoptosis and drug resistance in mammalian cells. It is a signaling adapter that couples activated growth factor receptors to signaling pathway. SHC1 expressed in neural stem cells but absent in mature neurons. Recombinant human SHC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHC1