SHC1 Antibody (15E11)

Product Discontinued

SHC1 Antibody (15E11) Summary

• Immunogen: Peptide corresponding to amino acids 314-316 of human p52 SHC.
• Specificity: phosphorylated SHC protein isoforms
  46,52,67kDa weight may be detected.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 2X PBS/PEG and Sucrose
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_003029

Alternate Names for SHC1 Antibody (15E11)

• FLJ26504
• p66
• p66SHC
• SH2 domain protein C1
• SHC (Src homology 2 domain containing) transforming protein 1
• SHC (Src homology 2 domain-containing) transforming protein 1
• SHC
• SHC1
• SHCA
• SHC-transforming protein 1
• SHC-transforming protein 3
• SHC-transforming protein A
• Src homology 2 domain-containing-transforming protein C1

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHC1
• Uniprot:
  • P29353()