SHARPIN Antibody (881816) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clone: 881816
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

SHARPIN Antibody (881816) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human SHARPIN
  Ala27-Ala182
  Accession # Q9H0F6
• Specificity: Detects human SHARPIN in ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHARPIN Antibody (881816) [Alexa Fluor® 488]

• DKFZp434N1923
• hSIPL1
• SHANK-associated RH domain interactor
• Shank-associated RH domain-interacting protein
• Shank-interacting protein-like 1
• SHARPIN
• SIPL1
• SIPL1DKFZP434N1923

Background

SHARPIN (Shank-associated RH Domain-interacting Protein), also called SIPL1 (Shank Interacting Protein-like 1) is an essential component of the cytosolic LUBAC ubiquitin conjugation complex. It conjugates linear ubiquitin chains to IKK gamma and activates NFk beta , thus regulating immune and inflammatory responses. SHARPIN is widely expressed, with upregulated expression in multiple tumor types. Human SHARPIN is a 387 amino acid (aa) protein containing self-association, SHANK1 interaction, and zinc finger domains. A 326 aa isoform diverges after aa 308 and lacks the zinc finger domain. Within aa 27-182, human SHARPIN shares 67% and 66% aa sequence identity with mouse and rat SHARPIN, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.