SHANK2 Antibody (711924) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: IHC
• Clone: 711924
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

SHANK2 Antibody (711924) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human SHANK2
  Lys849-Thr1029
  Accession # Q9UPX8
• Specificity: Detects human SHANK2 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHANK2 Antibody (711924) [Alexa Fluor® 350]

• AUTS17
• cortactin binding protein 1
• cortactin SH3 domain-binding protein
• Cortactin-binding protein 1
• CORTBP1
• CORTBP1CTTNBP1
• CTTNBP1
• GKAP/SAPAP interacting protein
• KIAA1022
• proline-rich synapse associated protein 1
• Proline-rich synapse-associated protein 1
• ProSAP1
• SH3 and multiple ankyrin repeat domains 2
• SH3 and multiple ankyrin repeat domains protein 2
• SHANK
• SHANK2
• SPANK-3

Background

SHANK2 (SH3 and multiple ANKyrin repeat domain protein 2; also CortBP1 and ProSAP1) is a 180-200 kDa member of the Shank family of proteins.  It is expressed in neurons and various epithelial cells, and serves as a multidomain scaffold for cell membrane proteins. For example, in the post-synaptic density of neurons, it provides support for the interaction between glutamate receptors and the actin cytoskeleton. In epithelia, SHANK2 regulates the trafficking and activity of Na/H Exchanger 3. Human SHANK2 is 1470 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 147-206), a PDZ domain (aa 247-341), a Pro-rich region (aa 509-535), a potential O-linked glycosylation site (aa 948-954) and a C-terminal SAM domain (aa 1407-1470). There are seven potential Ser/Thr phosphorylation sites. Multiple splice forms exist. A 220-240 kDa ankyrin repeat-containing isoform (SHANK2-E) possesses a 391 aa substitution for aa 1-12, and a 165 kDa isoform (potentially known as CortBP1) shows a 28 aa substitution for aa 1-238 coupled with a deletion of aa 383-390. Another potential isoform shows the previously noted 28 aa substitution for aa 1-238 coupled to a 19 aa substitution for aa 472-1470. Over aa 849-1029, human SHANK2 shares 80% aa sequence identity with mouse SHANK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.