SHANK1 Antibody (M369)

Western Blot: SHANK1 Antibody (M369) [NBP3-23391] - Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-SHANK1 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M369
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SHANK1 Antibody (M369) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M369 was generated from a sequence corresponding to amino acids in the C-terminal region of rat SHANK1. This sequence has high homology to human and mouse SHANK1. [UniProt# Q9WV48]
• Specificity: This antibody detects a 225 kDa protein corresponding to the molecular mass of Shank1 on SDS-PAGE immunoblots of adult mouse brain.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHANK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 225 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for SHANK1 Antibody (M369)

• SH3 and multiple ankyrin repeat domains 1
• SH3 and multiple ankyrin repeat domains protein 1
• Shank1
• Somatostatin receptor-interacting protein
• SSTR-interacting protein
• SSTRIPSPANK-1
• synamon

Background

Shank is a novel family of postsynaptic density proteins (PSD) that bind to PSD-95-associated protein GKAP. Its putative function is to act as a scaffold protein in the PSD to cross-link NMDA receptors/PSD-95 complexes and couple them to regulators of the actin cytoskeleton. Shank1a is a splice variant of Shank1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHANK1