SH3PX1 Antibody

Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody [NB100-2813] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Ca
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SH3PX1 Antibody Summary

• Immunogen: Peptide with sequence C-HKGAIEKVKESDK corresponding to internal region according to NP_057308.1.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNX9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Approx. 60 kDa band observed in human heart and human placenta lysates (calculated MW of 66.6 kDa band according to NP_057308.1). ICC/IF usage is reported in scientific literature (PMID: 23861900).
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SH3PX1 Overexpression Lysate
  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SH3PX1 Antibody

• MST155
• MSTP155
• Protein SDP1
• SDP1
• SH3 and PX domain-containing protein 1
• SH3PX1WISP
• SH3PXD3ASH3 and PX domain-containing protein SH3PX1
• sorting nexin 9
• sorting nexin-9
• WiskSH3 and PX domain-containing protein 3A

Background

The SH3PX1 (SNX1) gene is part of the sorting nexin family, thus plays a role in several states of intracellular trafficking. There is only one isoform at 595 amino acids long and at a mass of around 66 kDA. The SH3PX1 gene has been linked to choanal atresia, alcoholism, asthma, rabies, neuronitis, and wiskitt-aldrich syndrome. It is involved in clathrin derived and trans-Golgi network vesicle budding as well as membrane trafficking through interactions with other genes such as ITCH, DNM2, SYNJ1, ASAP1, and ADAM15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SH3PX1 Antibody (NB100-2813)(3)

Publications using NB100-2813

• Ma MP, Robinson PJ, Chircop M. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One. 2013-01-01 [PMID: 23861900] (ICC/IF, Human)
• Ma MP, Chircop M. SNX9, SNX18 and SNX33 are required for progression through and completion of mitosis. J Cell Sci. 2012-09-15 [PMID: 22718350] (Human)
• Soulet F, Yarar D, Leonard M, Schmid SL. SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis. Mol Biol Cell 2005-04-01 [PMID: 15703209]

Bioinformatics

• Gene Symbol: SNX9
• Entrez:
  • 51429(Human)
• Uniprot:
  • Q9Y5X1(Human)