SH3MD2 Antibody - BSA Free

Immunoprecipitation: SH3MD2 Antibody - BSA Free [NBP3-46932] - IP Result of anti-POSH (IP:, 4ug; Detection: 1:1000) with mouse brain 3000ug.

Western Blot: SH3MD2 Antibody - BSA Free [NBP3-46932] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (SH3MD2 antibody) at dilution of 1:1000

Immunohistochemistry: SH3MD2 Antibody - BSA Free [NBP3-46932] - Immunohistochemistry of paraffin-embedded human gliomas using (SH3MD2 antibody) at dilution of 1:100

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SH3MD2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SH3MD2 Antibody - BSA Free (NBP3-46932) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SH3 domain containing ring finger 1. (Uniprot# Q7Z6J0)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH3RF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:500-1:2000
  • Western Blot 1:500-1:2000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SH3MD2 Antibody - BSA Free

• EC 6.3.2
• EC 6.3.2.-
• KIAA1494SH3 multiple domains protein 2
• Plenty of SH3s
• POSHFLJ21602
• Protein POSH
• putative E3 ubiquitin-protein ligase SH3RF1
• RING finger protein 142
• RNF142plenty of SH3 domains
• SH3 domain containing ring finger 1
• SH3 domain-containing RING finger protein 1
• SH3 multiple domains 2
• SH3MD2

Background

SH3MD2 encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SH3RF1