SH3BP5 Antibody (2B3)

Western Blot: SH3BP5 Antibody (2B3) [H00009467-M01] - SH3BP5 monoclonal antibody (M01), clone 2B3 Analysis of SH3BP5 expression in A-431.

Immunocytochemistry/Immunofluorescence: SH3BP5 Antibody (2B3) [H00009467-M01] - Analysis of monoclonal antibody to SH3BP5 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SH3BP5 Antibody (2B3) [H00009467-M01] - Analysis of monoclonal antibody to SH3BP5 on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 1 ug/ml.

Western Blot: SH3BP5 Antibody (2B3) [H00009467-M01] - Reduced levels of the miR-132/212 targets Ache, MeCp2 and Pnkd in the bladder following outlet obstruction.Validated targets of miR-132/212 (including SH3BP5) were examined at the protein level using western blotting at various times after surgical obstruction of the urethra. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116784) licensed under a CC-BY license.

ELISA: SH3BP5 Antibody (2B3) [H00009467-M01] - Detection limit for recombinant GST tagged SH3BP5 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

SH3BP5 Antibody (2B3) Summary

• Immunogen: SH3BP5 (NP_004835, 4 a.a. ~ 112 a.a) partial recombinant protein with GST tag.GLEEEEEVDPRIQGELEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQR
• Localization: Mitochondrial
• Specificity: SH3BP5 - SH3-domain binding protein 5 (BTK-associated)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SH3BP5
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Immunoprecipitation was reported in scientific literature.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:25617893).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SH3BP5 Antibody (2B3)

• Sab
• SABSH3BP-5
• SH3 binding protein
• SH3 domain-binding protein 5
• SH3 domain-binding protein that preferentially associates with BTK
• SH3-domain binding protein 5 (BTK-associated)

Background

SH3BP5 (SH3 domain binding protein 5) binds to the SH3 domain of Bruton tyrosine kinase (BTK), a cytoplasmic tyrosine kinase crucial for the maturation of B-lineage cells. It inhibits the auto- and transphosphorylation activity of BTK and negatively regulates BTK-related cytoplasmic signaling in B-cells. SH3BP5 also interacts with JNK. The interaction with JNK occurs through a mitogen-activated protein KIM (kinase interaction motif) in a region distinct from the Bruton's tyrosine kinase-binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SH3BP5 Antibody (H00009467-M01)(9)

Publications using H00009467-M01

• Chu Q, Zhang Y, Zhong S, et al. N-n-Butyl Haloperidol Iodide Ameliorates Oxidative Stress in Mitochondria Induced by Hypoxia/Reoxygenation through the Mitochondrial c-Jun N-Terminal Kinase/Sab/Src/Reactive Oxygen Species Pathway in H9c2 Cells Oxidative Medicine and Cellular Longevity 2019-05-08 [PMID: 31205589] (WB, Rat)
• Paudel I, Hernandez SM, Portalatin GM et al. Sab Concentrations Indicate Chemotherapeutic Susceptibility in Ovarian Cancer Cell Lines. Biochem. J. 2018-10-15 [PMID: 30322886] (WB, Human)
• Chambers TP, Santiesteban L, Gomez D, Chambers JW. Sab mediates mitochondrial dysfunction involved in imatinib mesylate-induced cardiotoxicity. Toxicology. 2017-03-16 [PMID: 28315715]
• Chambers TP, Portalatin GM, Paudel I et al. Sub-chronic administration of LY294002 sensitizes cervical cancer cells to chemotherapy by enhancing mitochondrial JNK signaling Biochem. Biophys. Res. Commun. 2015-05-30 [PMID: 26032505] (WB, Human)
• Karbalaei M, Ekman M, Krawczyk K et al. Detrusor Induction of miR-132/212 following Bladder Outlet Obstruction: Association with MeCP2 Repression and Cell Viability PLoS One. 2015-01-24 [PMID: 25617893] (IF/IHC, Human, Mouse, Rat)
• Win S, Than TA, Fernandez-Checa JC, Kaplowitz N. JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death. Cell Death Dis. 2014-01-09 [PMID: 24407242]
• Chambers JW, Pachori A, Howard S et al. Inhibition of JNK Mitochondrial Localization and Signaling is Protective Against Ischemia-Reperfusion Injury in Rats J Biol Chem 2012-12-20 [PMID: 23258542] (IP, ICC/IF, WB, Human, Rat)
• Chambers JW, Howard S, Lograsso PV. Blocking c-Jun N-terminal Kinase (JNK) Translocation to the Mitochondria Prevents 6-Hydroxydopamine-induced Toxicity in Vitro and in Vivo J Biol Chem 2013-01-11 [PMID: 23184940] (WB, ICC/IF, Human)
• Chambers JW, Lograsso PV. Mitochondrial c-jun-N-terminal kinase (JNK) signaling initiates physiological changes resulting in amplification of reactive oxygen species generation. J Biol Chem. 2011-03-16 [PMID: 21454558]

Review for SH3BP5 Antibody (H00009467-M01) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Iru Paudel WB Human 04/14/2016

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: SH3BP5
• Entrez:
  • 9467(Human)
• OMIM:
  • 605612(Human)
• Uniprot:
  • NP_004835(Human)