Western Blot: SH3BP5 Antibody (2B3) [H00009467-M01] - SH3BP5 monoclonal antibody (M01), clone 2B3 Analysis of SH3BP5 expression in A-431.
Immunocytochemistry/ Immunofluorescence: SH3BP5 Antibody (2B3) [H00009467-M01] - Analysis of monoclonal antibody to SH3BP5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SH3BP5 Antibody (2B3) [H00009467-M01] - Analysis of monoclonal antibody to SH3BP5 on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 1 ug/ml.
Western Blot: SH3BP5 Antibody (2B3) [H00009467-M01] - Reduced levels of the miR-132/212 targets Ache, MeCp2 and Pnkd in the bladder following outlet obstruction.Validated targets of miR-132/212 (including SH3BP5) were ...read more
ELISA: SH3BP5 Antibody (2B3) [H00009467-M01] - Detection limit for recombinant GST tagged SH3BP5 is approximately 0.03ng/ml as a capture antibody.
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Immunoprecipitation was reported in scientific literature.
Read 1 Review rated 5 using H00009467-M01 in the following applications:
Mouse reactivity reported in scientific literature (PMID:25617893).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
In 1x PBS, pH 7.4
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for SH3BP5 Antibody (2B3)
SH3 binding protein
SH3 domain-binding protein 5
SH3 domain-binding protein that preferentially associates with BTK
SH3-domain binding protein 5 (BTK-associated)
SH3BP5 (SH3 domain binding protein 5) binds to the SH3 domain of Bruton tyrosine kinase (BTK), a cytoplasmic tyrosine kinase crucial for the maturation of B-lineage cells. It inhibits the auto- and transphosphorylation activity of BTK and negatively regulates BTK-related cytoplasmic signaling in B-cells. SH3BP5 also interacts with JNK. The interaction with JNK occurs through a mitogen-activated protein KIM (kinase interaction motif) in a region distinct from the Bruton's tyrosine kinase-binding domain.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.