SH3BP5 Antibody (2B3)

Images

 
Western Blot: SH3BP5 Antibody (2B3) [H00009467-M01] - SH3BP5 monoclonal antibody (M01), clone 2B3 Analysis of SH3BP5 expression in A-431.
Immunocytochemistry/ Immunofluorescence: SH3BP5 Antibody (2B3) [H00009467-M01] - Analysis of monoclonal antibody to SH3BP5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SH3BP5 Antibody (2B3) [H00009467-M01] - Analysis of monoclonal antibody to SH3BP5 on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 1 ug/ml.
Western Blot: SH3BP5 Antibody (2B3) [H00009467-M01] - Reduced levels of the miR-132/212 targets Ache, MeCp2 and Pnkd in the bladder following outlet obstruction.Validated targets of miR-132/212 (including SH3BP5) were ...read more
ELISA: SH3BP5 Antibody (2B3) [H00009467-M01] - Detection limit for recombinant GST tagged SH3BP5 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
2B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SH3BP5 Antibody (2B3) Summary

Immunogen
SH3BP5 (NP_004835, 4 a.a. ~ 112 a.a) partial recombinant protein with GST tag.GLEEEEEVDPRIQGELEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQR
Localization
Mitochondrial
Specificity
SH3BP5 - SH3-domain binding protein 5 (BTK-associated)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SH3BP5
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Immunoprecipitation was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 5
using
H00009467-M01 in the following applications:

Publications
Read Publications using
H00009467-M01 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:25617893).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SH3BP5 Antibody (2B3)

  • Sab
  • SABSH3BP-5
  • SH3 binding protein
  • SH3 domain-binding protein 5
  • SH3 domain-binding protein that preferentially associates with BTK
  • SH3-domain binding protein 5 (BTK-associated)

Background

SH3BP5 (SH3 domain binding protein 5) binds to the SH3 domain of Bruton tyrosine kinase (BTK), a cytoplasmic tyrosine kinase crucial for the maturation of B-lineage cells. It inhibits the auto- and transphosphorylation activity of BTK and negatively regulates BTK-related cytoplasmic signaling in B-cells. SH3BP5 also interacts with JNK. The interaction with JNK occurs through a mitogen-activated protein KIM (kinase interaction motif) in a region distinct from the Bruton's tyrosine kinase-binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SH3BP5 Antibody (H00009467-M01)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IP
(1)
WB
(5)
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Human
(5)
Mouse
(1)
Rat
(3)
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Showing Publications 1 - 9 of 9.
Publications using H00009467-M01 Applications Species
Chu Q, Zhang Y, Zhong S, et al. N-n-Butyl Haloperidol Iodide Ameliorates Oxidative Stress in Mitochondria Induced by Hypoxia/Reoxygenation through the Mitochondrial c-Jun N-Terminal Kinase/Sab/Src/Reactive Oxygen Species Pathway in H9c2 Cells Oxidative Medicine and Cellular Longevity May 8 2019 [PMID: 31205589] (WB, Rat) WB Rat
Paudel I, Hernandez SM, Portalatin GM et al. Sab Concentrations Indicate Chemotherapeutic Susceptibility in Ovarian Cancer Cell Lines. Biochem. J. Oct 15 2018 [PMID: 30322886] (WB, Human) WB Human
Chambers TP, Santiesteban L, Gomez D, Chambers JW. Sab mediates mitochondrial dysfunction involved in imatinib mesylate-induced cardiotoxicity. Toxicology. Mar 16 2017 [PMID: 28315715]
Chambers TP, Portalatin GM, Paudel I et al. Sub-chronic administration of LY294002 sensitizes cervical cancer cells to chemotherapy by enhancing mitochondrial JNK signaling Biochem. Biophys. Res. Commun. 2015 May 30 [PMID: 26032505] (WB, Human) WB Human
Karbalaei M, Ekman M, Krawczyk K et al. Detrusor Induction of miR-132/212 following Bladder Outlet Obstruction: Association with MeCP2 Repression and Cell Viability PLoS One. Jan 24 2015 [PMID: 25617893] (IHC, Human, Mouse, Rat) IHC Human, Mouse, Rat
Win S, Than TA, Fernandez-Checa JC, Kaplowitz N. JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death. Cell Death Dis. 2014 Jan 09 [PMID: 24407242]
Chambers JW, Pachori A, Howard S et al. Inhibition of JNK Mitochondrial Localization and Signaling is Protective Against Ischemia-Reperfusion Injury in Rats J Biol Chem 2012 Dec 20 [PMID: 23258542] (WB, IP, ICC/IF, Human, Rat) WB, IP, ICC/IF Human, Rat
Chambers JW, Howard S, Lograsso PV. Blocking c-Jun N-terminal Kinase (JNK) Translocation to the Mitochondria Prevents 6-Hydroxydopamine-induced Toxicity in Vitro and in Vivo J Biol Chem 2013 Jan 11 [PMID: 23184940] (WB, ICC/IF, Human) WB, ICC/IF Human
Chambers JW, Lograsso PV. Mitochondrial c-jun-N-terminal kinase (JNK) signaling initiates physiological changes resulting in amplification of reactive oxygen species generation. J Biol Chem. 2011 Mar 16. [PMID: 21454558]

Review for SH3BP5 Antibody (H00009467-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00009467-M01:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SH3BP5 H00009467-M01
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reviewed by:
Iru Paudel
WB Human 04/14/2016
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsLICOR blockin buffer, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionAnti-mouse
Secondary Manufacturer Cat#Cell signaling
Secondary Concentration1:10000

Details

Detection NotesFluorescence (LICOR)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SH3BP5 Antibody (H00009467-M01) (0)

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Recent Reviews

5
5
1
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1
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Iru Paudel
04/14/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol SH3BP5
Entrez
OMIM
Uniprot