Mouse sFRP-3/FRZB ELISA Kit (Colorimetric) Summary
| Description |
Assay Length: 1-5 hours |
| Specificity |
This assay has high sensitivity and excellent specificity for detection of Mouse FRZB. No significant cross-reactivity or interference between Mouse FRZB and analogues was observed. |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
50 - 100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
FRZB |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Alternate Names for Mouse sFRP-3/FRZB ELISA Kit (Colorimetric)
Background
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interactionwith Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appearsto be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bonedevelopment
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for sFRP-3/FRZB ELISA Kit (NBP2-77058). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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