SETDB2 Antibody (2F4) - Azide and BSA Free

Western Blot: SETDB2 Antibody (2F4) [H00083852-M17] - Analysis of SETDB2 expression in A-431 (Cat # L015V1).

• Reactivity: Hu
• Applications: WB, ELISA, ChIP
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SETDB2 Antibody (2F4) - Azide and BSA Free Summary

• Immunogen: SETDB2 (NP_114121.1, 451 a.a. ~ 550 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HPRTAKTEKCPPKFSNNPKELTMETKYDNISRIQYHSVIRDPESKTAIFQHNGKKMEFVSSESVTPEDNDGFKPPREHLNSKTKGAQKDSSSNHVDEFED
• Specificity: SET domain, bifurcated 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SETDB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in western blot and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SETDB2 Antibody (2F4) - Azide and BSA Free

• C13orf4
• chromosome 13 open reading frame 4
• chronic lymphocytic leukemia deletion region 8
• Chronic lymphocytic leukemia deletion region gene 8 protein
• CLLD8DKFZp761J1217
• DKFZp586I0123
• EC 2.1.1.43
• histone-lysine N-methyltransferase SETDB2
• KMT1FCLLL8
• Lysine N-methyltransferase 1F
• SET domain bifurcated 2
• SET domain, bifurcated 2

Background

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SETDB2 Antibody (H00083852-M17)(1)

Publication using H00083852-M17

• Lin CH, Wong SH, Kurzer JH et al. SETDB2 Links E2A-PBX1 to Cell-Cycle Dysregulation in Acute Leukemia through CDKN2C Repression. Cell Rep 2018-04-24 [PMID: 29694893]

Bioinformatics

• Gene Symbol: SETDB2
• Entrez:
  • 83852(Human)
• Uniprot:
  • NP_114121(Human)