Recombinant Human SET His Protein

SDS-Page: Recombinant Human SET Protein [NBP2-23464]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human SET His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-290 of Human SET
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SET
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SET His Protein

• 2PP2A
• HLA-DR-associated protein II
• I2PP2A
• I-2PP2A
• IGAAD
• Inhibitor of granzyme A-activated DNase
• inhibitor-2 of protein phosphatase-2A
• IPP2A2
• PHAPIITAF-I
• Phosphatase 2A inhibitor I2PP2A
• protein phosphatase type 2A inhibitor
• protein SET
• SET nuclear oncogene
• SET translocation (myeloid leukemia-associated)
• TAF-IBETA
• Template-activating factor I
• Template-Activating Factor-I, chromatin remodelling factor

Background

SET inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. Recombinant human SET protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for SET Recombinant Protein (NBP2-23464)(1)

Publication using NBP2-23464

• Zhang, L;Zhang, J;Xuan, X;Wu, D;Yu, J;Wang, P;Yang, X;Zhang, J;Gan, W;He, M;Liu, XM;Zhou, J;Wang, D;Gu, W;Li, D; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression Cell reports 2023-07-21 [PMID: 37480565] (Immunoprecipitation)

Bioinformatics

• Gene Symbol: SET