Septin-2 Antibody

Images

 
Western Blot: Septin-2 Antibody [NBP2-20301] - Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP2-20301] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Septin-2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Septin 2. The exact sequence is proprietary.
Localization
Cytoplasm; Spindle
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEPT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus tropicalis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Septin-2 Antibody

  • DIFF6NEDD-5
  • hNedd5
  • KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5
  • NEDD5Pnutl3
  • neural precursor cell expressed, developmentally down-regulated 5
  • septin 2
  • septin-2

Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEPT2
Uniprot