Septin-2 Antibody

Western Blot: Septin-2 Antibody [NBP2-20301] - Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP2-20301] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Septin 2 antibody (NBP2-20301) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin 2 antibody (NBP2-20301) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Septin-2 Antibody Summary

• Description: Novus Biologicals Rabbit Septin-2 Antibody (NBP2-20301) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Septin-2. The exact sequence is proprietary.
• Localization: Cytoplasm; Spindle
• Predicted Species: Mouse (99%), Rat (99%), Porcine (98%), Bovine (100%), Zebrafish (94%), Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPTIN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Septin-2 Antibody

• DIFF6NEDD-5
• hNedd5
• KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5
• NEDD5Pnutl3
• neural precursor cell expressed, developmentally down-regulated 5
• septin 2
• septin-2

Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SEPTIN2
• Uniprot:
  • Q15019(Human)