Septin-1 Antibody (1F12)

Images

 
Western Blot: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12. Lane 1: SEPT1 transfected lysatE (42 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of monoclonal antibody to SEPT1 on HeLa cell. Antibody concentration 10 ug/ml
Immunoprecipitation: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEPT1 rabbit polyclonal ...read more
Sandwich ELISA: Septin-1 Antibody (1F12) [H00001731-M03] - Detection limit for recombinant GST tagged SEPT1 is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, S-ELISA
Clone
1F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Septin-1 Antibody (1F12) Summary

Immunogen
SEPT1 (AAH12161, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEVTHDLLYEGYRARRLQSLARPGARDRASRSKLSRQSATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQRSQAQGEQSDAL
Specificity
SEPT1 - septin 1 (1F12)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SEPT1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Septin-1 Antibody (1F12)

  • DIFF6SEP1
  • differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase)
  • LARP
  • Peanut-like protein 3
  • PNUTL3MGC20394
  • septin 1
  • septin-1
  • Serologically defined breast cancer antigen NY-BR-24

Background

This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. This gene encodes a protein associated with the tau-based paired helical filament core, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SEPT1
Entrez
Uniprot