Semenogelin I Overexpression Lysate (Native)


Western Blot: Semenogelin I Overexpression Lysate (Adult Normal) [NBL1-15806] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Semenogelin I.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Semenogelin I Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC203322 and based on accession number NM_198139. The protein contains a C-terminal DDK tag.
Homo sapiens semenogelin I (SEMG1), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Semenogelin I

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Semenogelin I Overexpression Lysate (Native)

  • CT103
  • dJ172H20.2
  • FLJ78262
  • semen coagulating protein
  • semenogelin IMGC14719
  • semenogelin-1
  • SEMGcancer/testis antigen 103
  • SGI


The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (b) is shorter but has the same N- and C-termini compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Semenogelin I Lysate (NBL1-15806) (0)

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Video Protocols

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Contact Information

Product PDFs


Gene Symbol SEMG1

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