Semaphorin 5A Antibody (914419) [Unconjugated]

Semaphorin 5A was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Semaphorin 5A Monoclonal Antibody (Catalog # MAB5896) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 914419
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Semaphorin 5A Antibody (914419) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Semaphorin 5A
  Glu23-Thr765
  Accession # Q13591
• Specificity: Detects human Semaphorin 5A in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA5A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 5A Antibody (914419) [Unconjugated]

• FLJ12815
• sema domain, seven thrombospondin repeats (type 1 and type 1-like)
• Sema F
• SEMA5A
• SEMAF
• Semaphorin 5A
• semaphorin F
• semaphorin-5A
• Semaphorin-F
• semF
• transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Background

Semaphorin 5A (Sema5A, previously called SemaF) is a 140 kDa protein of the semaphorin family of axon guidance molecules (1‑4). Class 5 semaphorins are type I transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains
(1‑3). Sema5A is expressed developmentally in oligodendrocytes, neuroepithelial cells surrounding retinal axons, the base of limb buds, the cardiac atrial septum and endocardial cushions, and the mesoderm surrounding cranial vessels (4‑6). The human Sema5A cDNA encodes a 22 amino acid (aa) signal sequence, a 946 aa extracellular domain (ECD), a 22 aa transmembrane sequence and an 85 aa cytoplasmic portion. Within aa 23‑765, which includes the sema domain and four of the seven TSP repeats, human Sema5A shares 93% aa identity with corresponding mouse, rat, and canine sequences. Semaphorins typically transduce signals through transmembrane plexins (1, 2). The sema domain of Sema5A binds plexin B3, triggering signaling via HGF R/c-Met (7). Both Sema5A and plexin B3 are expressed postnatally during differentiation and migration of central nervous system oligodendrocytes. However, plexin B3 is not significantly expressed prenatally and therefore unlikely to be the Sema5A receptor during development (7, 8). The Sema5A TSP repeats interact with either heparin sulfate or chondroitin sulfate proteoglycans (HSPG, CSPG) (9). HSPG interaction promotes attraction, while CSPG interaction promotes repulsion and is essential for axon fasciculation, independent of plexin B3 (9, 10). Sema5A mutations have been implicated in the genetic syndrome, cri-du-chat, while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson’s (3, 11). Sema5A expression may be upregulated in metastatic cancer cells and downregulated in autism (12, 13).

1. Flannery, E. and M. Duman-Scheel (2009) Curr. Drug Targets 10:611.
2. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
3. Simmons, A.D. et al. (1998) Biochem. Biophys. Res. Commun. 242:685.
4. Oster, S.F. et al. (2003) Development 130:775.
5. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
6. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.
7. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
8. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
9. Kantor, D.B. et al. (2004) Neuron 44:961.
10. Hilario, J.D. et al. (2008) Dev. Biol. 326:190.
11. Lin, L. et al. (2009) Trends Neurosci. 32:142.
12. Pan, G-Q. et al. (2009) World J. Gastroenterol. 15:2800.
13. Melin, M. et al. (2006) Neuropsychobiology 54:64.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 5A Antibody (MAB5896)(1)

Publication using MAB5896

• M Lobna, E Nasren, S Adi, B Jacob, GA Maria, M Marcus, V Zahava The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A Biomolecules, 2021-03-02;11(3):. 2021-03-02 [PMID: 33801296] (IHC, Human)

Bioinformatics

• Gene Symbol: SEMA5A
• Uniprot:
  • Q13591(Human)