![Western Blot: SEL1L Antibody [NBP2-76965] - Western blot analysis of SeL1L on Zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SEL1L-Antibody-Western-Blot-NBP2-76965-img0001.jpg "Western Blot: SEL1L Antibody [NBP2-76965] - Western blot analysis of SeL1L on Zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
| Immunogen | Recombinant protein within zebrafish SEL1L aa 109-281 / 776. (SwissProt: K4FBI1 Zebrafish) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SEL1L |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.2% BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SEL1L |
![Immunocytochemistry/Immunofluorescence: SEL1L Antibody [NBP2-76965] - Immunofluorescence staining of paraffin-embedded zebrafish using anti-SEL1L rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SEL1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76965-img0002.jpg "Immunocytochemistry/Immunofluorescence: SEL1L Antibody [NBP2-76965] - Immunofluorescence staining of paraffin-embedded zebrafish using anti-SEL1L rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.")
![Immunohistochemistry: SEL1L Antibody [NBP2-76965] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SeL1L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SEL1L-Antibody-Immunohistochemistry-NBP2-76965-img0003.jpg "Immunohistochemistry: SEL1L Antibody [NBP2-76965] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SeL1L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.")
