SEC22B Antibody - Azide and BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SEC22B Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide from human SEC22B conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SEC22B
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 27319744).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for SEC22B Antibody - Azide and BSA Free

• ERS24
• ERS-24sec22b
• SEC22 vesicle trafficking protein homolog B (S. cerevisiae) (gene/pseudogene)
• SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
• SEC22 vesicle trafficking protein-like 1 (S. cerevisiae)
• SEC22 vesicle trafficking protein-like 1
• SEC22 vesicle-trafficking protein homolog B
• SEC22 vesicle-trafficking protein-like 1
• SEC22, vesicle trafficking protein (S. cerevisiae)-like 1
• SEC22L1ER-Golgi SNARE of 24 kDa
• vesicle-trafficking protein SEC22b

Background

The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. There is evidence for use of multiple polyadenylation sites for the transcript. FUNCTION: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER. SUBUNIT: Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex. SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEC22B Antibody (NB100-98766)(1)

Publication using NB100-98766

• Dai J, Lu Y, Wang C et al. Vps33b regulates Vwf-positive vesicular trafficking in megakaryocytes. J. Pathol. 2016-06-19 [PMID: 27319744] (WB, ICC/IF, Human, Mouse)

Bioinformatics

• Gene Symbol: SEC22B
• Entrez:
  • 9554(Human)
  • 20333(Mouse)
  • 310710(Rat)
• Uniprot:
  • O75396(Human)