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Membrin Antibody (4HAD6)

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Western Blot: Membrin Antibody (4HAD6) [NBP2-89025] - Western blot analysis of VAM-PT046: Lane 1: MW marker, Lane 2: HeLa, Lane 3: PC-12, Lane 4: RK13, Lane 5: Vero.
Western Blot: Membrin Antibody (4HAD6) [NBP2-89025] - Western blot analysis of VAM-PT046: Lane 1: MW marker, Lane 2: HeLa, Lane 3: PC-12, Lane 4: RK13, Lane 5: Vero.
Immunohistochemistry: Membrin Antibody (4HAD6) [NBP2-89025] - Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Mouse Monoclonal Antibody (MSN/492).

Product Details

Summary
Reactivity Hu, Rt, Po, Ca, Ch, Ha, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4HAD6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Membrin Antibody (4HAD6) Summary

Immunogen
Recombinant rat Membrinprotein with a His tag.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GOSR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS , pH 7.2, containing 0.1mM PMSF and 50% glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Membrin Antibody (4HAD6)

  • golgi SNAP receptor complex member 2
  • GS27Bos1,27 kDa Golgi SNARE protein
  • membrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western lane view shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Syntaxin 5 at approximately 39 and 47 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Syntaxin 5 at approximately 35 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Syntaxin 5 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot: VTI1B Antibody [NBP2-33414] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: VTI1B Antibody [NBP2-33414] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunocytochemistry/Immunofluorescence: VTI1B Antibody [NBP2-33414] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
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Western Blot: NSF Antibody [NBP1-87035] - Analysis in human cell line A-549.
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Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in control (vector only transfected HEK293T lysate) and SNAP25 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Azide and BSA Free
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Immunohistochemistry-Paraffin: SCFD1 Antibody (5G4) [NBP2-02031] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SCFD1 mouse monoclonal antibody.
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Membrin Antibody
(4HAD6)
NBP2-89025
Membrin Antibody (4HAD6)
Species: Hu, Rt, Po, Ca, Ch, Ha, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol GOSR2