SEC13 Antibody (1280A) [Unconjugated]

Western blot shows lysates of human kidney tissue, human testis tissue, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 1280A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SEC13 Antibody (1280A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human SEC13
  Gly123-Gln322
  Accession # P55735
• Specificity: Detects human SEC13 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 0.3-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SEC13 Antibody (1280A) [Unconjugated]

• D3S1231E
• D3S1231ESEC13L1npp-20
• Npp-20
• protein SEC13 homolog
• SEC13 (S. cerevisiae)-like 1
• SEC13 homolog (S. cerevisiae)
• SEC13
• SEC13L1
• SEC13-like 1 isoform
• SEC13-like protein 1
• SEC13R
• SEC13-related protein
• SEC13RSEC13-like 1 (S. cerevisiae)

Background

Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p^.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport.  Presenillin-1, which is implicated in early-onset Alzheimer's disease has been shown to interact with Sec13 vesicles.  Additionally, Sec13 has been shown to be a component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the GATOR2 sub-complex of the GATOR complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEC13 Antibody (MAB9055)(2)

Publications using MAB9055

• A El-Gazzar, B Voraberger, F Rauch, M Mairhofer, K Schmidt, B Guillemyn, G Mitulovi?, V Reiterer, M Haun, MM Mayr, JA Mayr, S Kimeswenge, O Drews, V Saraff, N Shaw, N Fratzl-Zel, S Symoens, H Farhan, W Högler Bi-allelic mutation in SEC16B alters collagen trafficking and increases ER stress Embo Molecular Medicine, 2023-03-14;0(0):e16834. 2023-03-14 [PMID: 36916446]
• Olga Shomron, Inbar Nevo-Yassaf, Tamar Aviad, Yakey Yaffe, Eitan Erez Zahavi, Anna Dukhovny et al. COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers Journal of Cell Biology 2021-06-07 [PMID: 33852719]

Bioinformatics

• Uniprot:
  • P55735()