SDNSF/MCFD2 Antibody (294301) [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 294301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SDNSF/MCFD2 Antibody (294301) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SDNSF/MCFD2
  Glu27-Gln146
  Accession # Q8NI22
• Specificity: Detects human SDNSF/MCFD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SDNSF is observed. In Western blots, no cross-reactivity with recombinant mouse SDNSF is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCFD2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDNSF/MCFD2 Antibody (294301) [Unconjugated]

• DKFZp686G21263
• F5F8D2
• LMAN1IP
• MCFD2
• multiple coagulation factor deficiency 2
• multiple coagulation factor deficiency protein 2
• neural stem cell derived neuronal survival protein
• Neural stem cell-derived neuronal survival protein
• SDNSF
• SDNSFF5F8D

Background

SDNSF, also known as MCFD2 (Multiple Coagulation Factor Deficiency 2), was first identified as a secreted molecule that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of a complex that transports proteins from the ER to the Golgi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDNSF/MCFD2 Antibody (MAB2357)(1)

Publication using MAB2357

• H Yagi, M Yagi-Utsum, R Honda, Y Ohta, T Saito, M Nishio, S Ninagawa, K Suzuki, T Anzai, Y Kamiya, K Aoki, M Nakanishi, T Satoh, K Kato Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor Nat Commun, 2020-03-13;11(1):1368. 2020-03-13 [PMID: 32170195] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MCFD2
• Uniprot:
  • Q8NI22()