SDHA Antibody (1084056) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 1084056
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

SDHA Antibody (1084056) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects a synthetic peptide corresponding to residues near amino acid 610 of human SDHA protein by Direct ELISA.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDHA Antibody (1084056) [Alexa Fluor™ Plus 647]

• CMD1GG
• flavoprotein subunit of complex II
• FP
• MC2DN1
• NDAXOA
• PGL5
• PPGL5
• SDH1
• SDH2
• SDHA
• SDHF
• succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

SDHA is 73 kDa protein located on the inner membrane of the mitochondria which participates in both the citric acid cycle and the respiratory chain. SDHA is one subunit of the SDH complex, which contains 4 total subunits, that are encoded by nuclear genes.  All the SDH genes are involved in the tumorigenesis of different types of cancers. Germline SDHA mutations are a driver for multiple types of cancers.

1. Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S. Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction. J Biol Chem. 2006 Mar 17;281(11):7309-16. doi: 10.1074/jbc.M508173200. Epub 2005 Dec 27. PMID: 16407191. 
2. Schipani A, Nannini M, Astolfi A, Pantaleo MA. SDHA Germline Mutations in SDH-Deficient GISTs: A Current Update. Genes (Basel). 2023 Mar 4;14(3):646. doi: 10.3390/genes14030646. PMID: 36980917; PMCID: PMC10048394.
3. Dubard Gault M, Mandelker D, DeLair D, Stewart CR, Kemel Y, Sheehan MR, Siegel B, Kennedy J, Marcell V, Arnold A, Al-Ahmadie H, Modak S, Robson M, Shukla N, Roberts S, Vijai J, Topka S, Kentsis A, Cadoo K, Carlo M, Latham Schwark A, Reznik E, Dinatale R, Hechtman J, Borras Flores E, Jairam S, Yang C, Li Y, Bayraktar EC, Ceyhan-Birsoy O, Zhang L, Kohlman W, Schiffman J, Stadler Z, Birsoy K, Kung A, Offit K, Walsh MF. Germline SDHA mutations in children and adults with cancer. Cold Spring Harb Mol Case Stud. 2018 Aug 1;4(4):a002584. doi: 10.1101/mcs.a002584. PMID: 30068732; PMCID: PMC6071569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.