SCO1 Knockout 293T Cell Lysate

Images

 
There are currently no images for SCO1 Lysate (NBP2-65508).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

SCO1 Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 1 and 1 bp insertion in exon 1
Gene
SCO1

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SCO1 Knockout 293T Cell Lysate

  • protein SCO1 homolog, mitochondrial
  • SCO cytochrome oxidase deficient homolog 1 (yeast)
  • SCOD1SCO (cytochrome oxidase deficient, yeast) homolog 1

Background

Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56503
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
NBP1-77274
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, IF
NBP2-30388
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32550
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-59438
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-65875
Species: Hu
H00001353-B02P
Species: Hu
Applications: WB, ICC/IF
NBP1-86322
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-20537
Species: Hu
Applications: WB
NBP2-66995
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-59376
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP
NBP1-88678
Species: Hu
Applications: IHC, IHC-P
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
H00009973-M03
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB100-64775
Species: Hu, Bb, Bv, Pm
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, IHC-P (-)
NBP1-59560
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ye, Ze
Applications: WB, IHC, IHC-P
NBP2-52983
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-360
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-65508
Species: Hu
Applications: WB

Publications for SCO1 Lysate (NBP2-65508) (0)

There are no publications for SCO1 Lysate (NBP2-65508).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SCO1 Lysate (NBP2-65508) (0)

There are no reviews for SCO1 Lysate (NBP2-65508). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SCO1 Lysate (NBP2-65508) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SCO1 Products

Bioinformatics Tool for SCO1 Lysate (NBP2-65508)

Discover related pathways, diseases and genes to SCO1 Lysate (NBP2-65508). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SCO1 Lysate (NBP2-65508)

Discover more about diseases related to SCO1 Lysate (NBP2-65508).
 

Pathways for SCO1 Lysate (NBP2-65508)

View related products by pathway.

PTMs for SCO1 Lysate (NBP2-65508)

Learn more about PTMs related to SCO1 Lysate (NBP2-65508).

Blogs on SCO1

There are no specific blogs for SCO1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SCO1 Knockout 293T Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SCO1