SCF/c-kit Ligand Antibody (SC61-06)

Western Blot: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Western blot analysis of SCF/c-kit Ligand on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Go

Immunocytochemistry/Immunofluorescence: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Staining SCF in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Analysis of paraffin-embedded human kidney tissue using anti-SCF antibody. Counter stained with hematoxylin.

Flow Cytometry: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Analysis of MCF-7 cells with SCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Analysis of SCF on Raji cells lysates using anti-SCF antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Staining SCF in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Staining SCF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SCF/c-kit Ligand Antibody (SC61-06) [NBP2-67622] - Analysis of paraffin-embedded human colon cancer tissue using anti-SCF antibody. Counter stained with hematoxylin.

Product Discontinued

SCF/c-kit Ligand Antibody (SC61-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SCF/c-kit Ligand aa 36-85 / 273. (SwissProt: P21583 Human)
• Localization: Cell membrane, Cytoplasm, Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KITLG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SCF/c-kit Ligand Antibody (SC61-06)

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCF/c-kit Ligand Antibody (NBP2-67622)(1)

Publication using NBP2-67622

• van Beelen ESA, van der Valk WH, de Groot JCMJ et al. Migration and fate of vestibular melanocytes during the development of the human inner ear Dev Neurobiol 2020-10-19 [PMID: 33075185] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: KITLG