SCARA5 Antibody (462002) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: IHC
• Clone: 462002
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

SCARA5 Antibody (462002) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SCARA5
  Arg83-Pro491
  Accession # Q8K299
• Specificity: Detects mouse SCARA5 in direct ELISAs. In direct ELISAs, 100% cross-reactivty with recombinant human SCARA5 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCARA5 Antibody (462002) [Alexa Fluor® 594]

• FLJ23907
• MGC45780
• SCARA5
• scavenger receptor class A, member 5 (putative)
• Tesr
• testis expressed scavenger receptor
• UNQ2938/PRO28700

Background

Scavenger receptor A5 (SCARA5; also known as testis expressed scavenger receptor Tesr) is a Class A scavenger receptor that is related to CL-P1, MARCO, SCARA3, and SR-A1. All are type II transmembrane proteins that contain a collagenous stalk (1‑3). Mature mouse SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 410 aa C-terminal extracellular domain (ECD) that contains a coiled-coil, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) region (4, 5). Within the ECD, mouse SCARA5 shares 87% and 98% aa sequence identity with human and rat SCARA5, respectively. It shares 24%‑29% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternative splicing generates one isoform that lacks the SRCR domain and a second that additionally lacks the cytoplasmic and transmembrane regions. SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8K299()