SART1 Antibody

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Western Blot: SART1 Antibody [NB100-1145] - NB100-1145 (0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SART1 Antibody Summary

Immunogen
Peptide with sequence GSSKKHRGEKEAA-C corresponding to N-Terminus according to NP_005137.1.
Localization
Cytoplasmic and Nuclear
Specificity
This antibody is expected to recognise both the human SART1(800) and SART1(259) proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SART1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 125 kDa band observed in HeLa lysates (calculated MW of 90.3 kDa band according to NP_005137) and is in agreement with scientific literature (Vertegaal et al).
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-1145.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SART1 Antibody

  • Allergen Hom s 1
  • Ara1
  • HAF
  • HOMS1
  • hSART-1
  • hSnu66
  • hypoxia-associated factor
  • IgE Autoantigen
  • MGC2038
  • SART1
  • SART-1
  • SART1(259) protein
  • SART1(800) protein
  • SART1259
  • small nuclear ribonucleoprotein 110kDa (U4/U6.U5)
  • SNRNP110
  • SNU66 homolog
  • Snu66
  • squamous cell carcinoma antigen recognised by T cells
  • Squamous cell carcinoma antigen recognized by T cells 1
  • squamous cell carcinoma antigen recognized by T cells
  • U4/U6.U5 tri-snRNP-associated 110 kDa protein
  • U4/U6.U5 tri-snRNP-associated protein 1

Background

SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SART1 Antibody (NB100-1145)(1)


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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SART1
Entrez
Uniprot