Recombinant Human SAR1 His Protein

SDS-Page: Recombinant Human SAR1 His Protein [NBP1-30177] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human SAR1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 198 of Human SAR1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SAR1A
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 24.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SAR1 His Protein

• COPII-associated small GTPase
• EC 3.6.5
• masra2
• SAR1 gene homolog A
• SAR1 homolog A (S. cerevisiae)
• SAR1a gene homolog (S. cerevisiae) 1
• SAR1a gene homolog 1 (S. cerevisiae)
• SAR1a gene homolog 1
• SAR1SARA1GTP-binding protein SAR1a
• Sara

Background

SAR1a gene homolog 1, also known as SAR1A, belongs to the small GTPase superfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. GDP-bound Sar1 interacts with the membrane-bound exchange factor Sec12 and exchanges its bound GDP for GTP. Recombinant human SAR1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SAR1A