Recombinant Human S1P5/EDG-8 GST (N-Term) Protein

Images

 
12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA, AP

Order Details

Recombinant Human S1P5/EDG-8 GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-398 of Human EDG8 full-length ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MESGLLRPAPVSEVIVLHYNYTGKLRGARYQPGAGLRADAVVCLAVCAFIVLENLAVLLVLGRHPRFHAPMFLLLGSLTLSDLLAGAAYAANILLSGPLTLKLSPALWFAREGGVFVALTASVLSLLAIALERSLTMARRGPAPVSSRGRTLAMAAAAWGVSLLLGLLPALGWNCLGRLDACSTVLPLYAKAYVLFCVLAFVGILAAICALYARIYCQVRANARRLPARPGTAGTTSTRARRKPRSLALLRTLSVVLLAFVACWGPLFLLLLLDVACPARTCPVLLQADPFLGLAMANSLLNPIIYTLTNRDLRHALLRLVCCGRHSCGRDPSGSQQSASAAEASGGLRRCLPPGLDGSFSGSERSSPQRDGLDTSGSTGSPGAPTAARTLVSEPAAD

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Full Length Recombinant Protein
Gene
S1PR5
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
68.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human S1P5/EDG-8 GST (N-Term) Protein

  • EDG8
  • Edg-8
  • EDG8S1P receptor Edg-8
  • Endothelial differentiation G-protein-coupled receptor 8
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 8
  • LPB4
  • S1P receptor 5
  • S1P5
  • S1PR5
  • sphingosine 1-phosphate receptor 5
  • sphingosine 1-phosphate receptor EDG8
  • Sphingosine 1-phosphate receptor Edg-8
  • sphingosine-1-phosphate receptor 5
  • SPPR-1
  • SPPR-2

Background

The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1; MIM 601974) are S1P receptors; others (e.g., EDG2; MIM 602282) are LPA receptors.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB7089
Species: Mu
Applications: CyTOF-ready, Flow, IHC
NBP3-05160
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-26691
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24762
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-24500
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-03363
Species: Ch, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-37477
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NLS1017
Species: Bt, Bv, Ca, Eq, Hu, Mu
Applications: IHC, IHC-P
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
AF6396
Species: Hu
Applications: WB
NLS1895
Species: Bt, Ha, Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P
NBP1-43299
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-71867
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NLS426
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
DGD150
Species: Hu
Applications: ELISA

Publications for S1P5/EDG-8 Recombinant Protein (H00053637-P01) (0)

There are no publications for S1P5/EDG-8 Recombinant Protein (H00053637-P01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for S1P5/EDG-8 Recombinant Protein (H00053637-P01) (0)

There are no reviews for S1P5/EDG-8 Recombinant Protein (H00053637-P01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for S1P5/EDG-8 Recombinant Protein (H00053637-P01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional S1P5/EDG-8 Products

Bioinformatics Tool for S1P5/EDG-8 Recombinant Protein (H00053637-P01)

Discover related pathways, diseases and genes to S1P5/EDG-8 Recombinant Protein (H00053637-P01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for S1P5/EDG-8 Recombinant Protein (H00053637-P01)

Discover more about diseases related to S1P5/EDG-8 Recombinant Protein (H00053637-P01).
 

Pathways for S1P5/EDG-8 Recombinant Protein (H00053637-P01)

View related products by pathway.

PTMs for S1P5/EDG-8 Recombinant Protein (H00053637-P01)

Learn more about PTMs related to S1P5/EDG-8 Recombinant Protein (H00053637-P01).
 

Research Areas for S1P5/EDG-8 Recombinant Protein (H00053637-P01)

Find related products by research area.

Blogs on S1P5/EDG-8

There are no specific blogs for S1P5/EDG-8, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human S1P5/EDG-8 GST (N-Term) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol S1PR5