Mouse S1P5/EDG-8 ELISA Kit (Colorimetric)

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ELISA: Mouse S1P5/EDG-8 ELISA Kit (Colorimetric) [NBP2-82430] - Samples were spiked with high concentrations of Mouse S1P5/EDG-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Mouse S1P5/EDG-8 ELISA Kit (Colorimetric) [NBP2-82430] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

Mouse S1P5/EDG-8 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse S1P5/EDG-8 in samples. No Significant cross-reactivity or interference between Mouse S1P5/EDG-8 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.96
Intra-Assay
CV% < 5.34
Spike Recovery
84-104%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
S1PR5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse S1P5/EDG-8 ELISA Kit (Colorimetric)

  • EDG8
  • Edg-8
  • EDG8S1P receptor Edg-8
  • Endothelial differentiation G-protein-coupled receptor 8
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 8
  • LPB4
  • S1P receptor 5
  • S1P5
  • S1PR5
  • sphingosine 1-phosphate receptor 5
  • sphingosine 1-phosphate receptor EDG8
  • Sphingosine 1-phosphate receptor Edg-8
  • sphingosine-1-phosphate receptor 5
  • SPPR-1
  • SPPR-2

Background

Edg8 is a Lysophospholipid/Lysosphingolipid Receptor activated by sphingosine-1-phosphate. Two differently expressed splice variants, 2.4 kb and 5.4 kb, are produced by alternative splicing. Edg8 has been reported in rat brain, lung, small intestine, spinal cord, and spleen. ESTs have been isolated from human cancer libraries of the head/neck and skin, as well as mouse brain and liver libraries. Caution: The rat EDG-8 gene has also been described as NRG-1, but should not be confused with the neuregulin-1 gene whose approved symbol is NRG-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol S1PR5