S1P5/EDG-8 Antibody (282503) [Phycoerythrin]

HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 was stained with Mouse Anti-Human S1P5/EDG-8 PE-conjugated Monoclonal Antibody (Catalog # FAB3964P) and either (A) Mouse Anti-Human S1P5/EDG-8 PE-conjugated Monoclonal Antibody (Catalog # FAB3964P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP was stained with either (A) Mouse Anti-Human S1P5/EDG-8 PE-conjugated Monoclonal Antibody (Catalog # FAB3964P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

S1P5/EDG-8 Antibody (282503) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human S1P₅/EDG‑8
  Met1-Asp398
  Accession # Q9H228
• Specificity: Detects human and mouse S1P₅/EDG‑8 in flow cytometry. Stains human and mouse S1P₅/EDG‑8 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: S1PR5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P5/EDG-8 Antibody (282503) [Phycoerythrin]

• EDG8
• Edg-8
• EDG8S1P receptor Edg-8
• Endothelial differentiation G-protein-coupled receptor 8
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 8
• LPB4
• S1P receptor 5
• S1P5
• S1PR5
• sphingosine 1-phosphate receptor 5
• sphingosine 1-phosphate receptor EDG8
• Sphingosine 1-phosphate receptor Edg-8
• sphingosine-1-phosphate receptor 5
• SPPR-1
• SPPR-2

Background

S1P₅ is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P₅ is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₅ show 96% and 97% aa identity with mouse and rat S1P₅, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P₅ is upregulated in large granular lymphocytic leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S1PR5
• Uniprot:
  • Q9H228()