S1P4/EDG-6 Overexpression Lysate (Native)

Images

 
Western Blot: EDG6 Overexpression Lysate (Adult Normal) [NBL1-15668] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

S1P4/EDG-6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204887 and based on accession number NM_003775. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sphingosine-1-phosphate receptor 4 (S1PR4), mRNA.
Gene
S1PR4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for S1P4/EDG-6 Overexpression Lysate (Native)

  • EDG6
  • EDG6S1P receptor Edg-6
  • Endothelial differentiation G-protein coupled receptor 6
  • endothelial differentiation, G-protein-coupled receptor 6
  • endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor6,endothelial differentiation, G protein coupled receptor 6
  • S1P receptor 4
  • S1P4
  • S1P4LPC1
  • S1PR4
  • SLP4
  • sphingosine 1-phosphate receptor 4
  • Sphingosine 1-phosphate receptor Edg-6
  • sphingosine-1-phosphate receptor 4

Background

This gene is a member of the endothelial differentiation, G-protein-coupled (EDG)) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands, and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for S1P4/EDG-6 Lysate (NBL1-15668) (0)

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Bioinformatics

Gene Symbol S1PR4
Entrez