S1P2/EDG-5/S1PR2 Antibody (368510) [Allophycocyanin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human S1P2/EDG-5 APC-conjugated Monoclonal Antibody (Catalog # FAB3649A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 APC-conjugated Monoclonal Antibody (Catalog # FAB3649A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

S1P2/EDG-5/S1PR2 Antibody (368510) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human S1P₂/EDG-5
  Met1-Val353
  Accession # AAC98919
• Specificity: Detects human S1P₂/EDG-5. Stains human S1P₂/EDG-5 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S1PR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P2/EDG-5/S1PR2 Antibody (368510) [Allophycocyanin]

• AGR16
• EDG5
• EDG-5
• endothelial differentiation G-protein coupled receptor 5
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
• GPCR13
• GPCR18
• H218
• LPB2
• S1P receptor 2
• S1P receptor EDG5
• S1P receptor Edg-5
• S1P2
• S1PR2
• sphingosine 1-phosphate receptor 2
• sphingosine 1-phosphate receptor Edg-5
• sphingosine-1-phosphate receptor 2

Background

S1P₂ (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a 353 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₂ show 82.5% aa identity with mouse S1P₂ and 92% aa identity with human EDG-3, the most closely related family member. Both are expressed predominantly in the lung, heart, kidney, liver, spleen, thymus, testis and brain, mediating both mitogenic and anti-apoptotic effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S1PR2
• Uniprot:
  • AAC98919()