Recombinant Human RSK2 Protein

HPLC: Recombinant RSK2 Protein [NBP1-99502]

Product Discontinued

Recombinant Human RSK2 Protein Summary

• Description: Recombinant protein for Human RSK2
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_004586)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60881)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: RPS6KA3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: The specific activity of RSK2 was determined to be 157 nmol /min/mg as per activity assay protocol.
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human RSK2 Protein

• 90 kDa ribosomal protein S6 kinase 3
• CLS
• Coffin-Lowry syndrome
• EC 2.7.11
• EC 2.7.11.1
• HU-3
• Insulin-stimulated protein kinase 1
• ISPK1
• ISPK-1
• MAP kinase-activated protein kinase 1b
• MAPK-activated protein kinase 1b
• MAPKAP kinase 1b
• MAPKAPK1B
• MAPKAPK-1b
• mental retardation, X-linked 19
• MRX19
• p90-RSK2
• p90RSK3
• pp90RSK2
• ribosomal protein S6 kinase alpha-3
• ribosomal protein S6 kinase, 90kD, polypeptide 3
• ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Ribosomal S6 kinase 2
• RPS6KA3
• RSK
• RSK2
• RSK-2
• RSK2p90-RSK 3
• S6K-alpha3
• S6K-alpha-3

Background

RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine/threonine kinases. RSK2 has been shown to mediate growth factor signaling via RAS and MAPK leading to the induction of CREB serine-133 phosphorylation and activation of gene expression (1). Mutations in RSK2 have been shown to be responsible for Coffin-Lowry syndrome (CLS) which is a X-linked disorder characterized by severe psychomotor retardation, facial and digital dysmorphisms, and progressive skeletal deformations (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA3