RSK2 Antibody (4E10)

Western Blot: RSK2 Antibody (4E10) [NBP2-37272] - Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: RSK2 Antibody (4E10) [NBP2-37272] - Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: RSK2 Antibody (4E10) [NBP2-37272] - Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and negative control (red).

Western Blot: RSK2 Antibody (4E10) [NBP2-37272] - Western blot analysis using RSK2 mAb against human RSK2 (AA: 1-212) recombinant protein. (Expected MW is 49.7 kDa)

ELISA: RSK2 Antibody (4E10) [NBP2-37272] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

RSK2 Antibody (4E10) Summary

• Immunogen: Purified recombinant fragment of human RSK2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KA3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for RSK2 Antibody (4E10)

• 90 kDa ribosomal protein S6 kinase 3
• CLS
• Coffin-Lowry syndrome
• EC 2.7.11
• EC 2.7.11.1
• HU-3
• Insulin-stimulated protein kinase 1
• ISPK1
• ISPK-1
• MAP kinase-activated protein kinase 1b
• MAPK-activated protein kinase 1b
• MAPKAP kinase 1b
• MAPKAPK1B
• MAPKAPK-1b
• mental retardation, X-linked 19
• MRX19
• p90-RSK2
• p90RSK3
• pp90RSK2
• ribosomal protein S6 kinase alpha-3
• ribosomal protein S6 kinase, 90kD, polypeptide 3
• ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Ribosomal S6 kinase 2
• RPS6KA3
• RSK
• RSK2
• RSK-2
• RSK2p90-RSK 3
• S6K-alpha3
• S6K-alpha-3

Background

Ribosomal protein S6 kinase II (RSK2) is a protein serine/threonine kinase that was originally detected by its ability to catalyze the multi-site phosphorylation of the 40S ribosomal protein S6 in vitro. One of the important substrates of RSK may be the Glycogenbinding subunit of Protein phosphatase-1, which when phosphorylated by RSK, appears to activate Protein phosphatase 1. This results in increased dephosphorylation and activation of Glycogen synthase to enhance Glycogen synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA3