RPS3 Antibody (SA46-08)

Western Blot: RPS3 Antibody (SA46-08) [NBP2-67301] - Western blot analysis of RPS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunocytochemistry/Immunofluorescence: RPS3 Antibody (SA46-08) [NBP2-67301] - Staining RPS3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPS3 Antibody (SA46-08) [NBP2-67301] - Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 mi

Western Blot: RPS3 Antibody (SA46-08) [NBP2-67301] - Analysis of RPS3 on PC12 cell lysates using anti-RPS3 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: RPS3 Antibody (SA46-08) [NBP2-67301] - Staining RPS3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPS3 Antibody (SA46-08) [NBP2-67301] - Analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RPS3 Antibody (SA46-08) [NBP2-67301] - Analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RPS3 Antibody (SA46-08) [NBP2-67301] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30

Product Discontinued

RPS3 Antibody (SA46-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human RPS3 aa 125-174 / 243. (SwissProt: P23396 Human; SwissProt: P62908 Mouse; SwissProt: P62909 Rat)
• Localization: Cytoplasm, Nucleus, Mitochondrion inner membrane
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RPS3 Antibody (SA46-08)

• 40S ribosomal protein S3
• FLJ26283
• FLJ27450
• IMR-90 ribosomal protein S3
• MGC87870
• ribosomal protein S3

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS3