RPS18 Overexpression Lysate (Native)

Images

 
Western Blot: RPS18 Overexpression Lysate (Adult Normal) [NBL1-15563] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPS18.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RPS18 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219170 and based on accession number NM_022551. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ribosomal protein S18 (RPS18), mRNA.
Gene
RPS18

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RPS18

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RPS18 Overexpression Lysate (Native)

  • D6S218E
  • HKE3
  • KE3
  • KE-3
  • Ke-3,40S ribosomal protein S18
  • MGC117351
  • MGC126835
  • MGC126837
  • rhabdomyosarcoma antigen MU-RMS-40.21
  • ribosomal protein S18

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1595
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP2-33717
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87799
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13262
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-58885
Species: Hu
Applications: WB, ICC/IF
NBP1-82761
Species: Hu
Applications: IHC, IHC-P
H00006192-P01
Species: Hu
NBP1-80959
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80961
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
H00006201-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-92350
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-87100
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87797
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-57365
Species: Hu
Applications: WB
AF6574
Species: Mu
Applications: WB
NBP2-38612
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83254
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00006193-M02
Species: Hu, Mu, Rt, Pr
Applications: WB, ELISA, ICC/IF
NB100-56350
Species: Hu, Mu, Rt
Applications: WB

Publications for RPS18 Lysate (NBL1-15563) (0)

There are no publications for RPS18 Lysate (NBL1-15563).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RPS18 Lysate (NBL1-15563) (0)

There are no reviews for RPS18 Lysate (NBL1-15563). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RPS18 Lysate (NBL1-15563) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RPS18 Products

Bioinformatics Tool for RPS18 Lysate (NBL1-15563)

Discover related pathways, diseases and genes to RPS18 Lysate (NBL1-15563). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RPS18 Lysate (NBL1-15563)

Discover more about diseases related to RPS18 Lysate (NBL1-15563).
 

Pathways for RPS18 Lysate (NBL1-15563)

View related products by pathway.

PTMs for RPS18 Lysate (NBL1-15563)

Learn more about PTMs related to RPS18 Lysate (NBL1-15563).

Blogs on RPS18

There are no specific blogs for RPS18, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CD20 Antibody (AISB12)
NBP1-43435-0.1mg
RPS18 Antibody
NBP2-13263
5-HT1B Antibody
NB100-56350

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RPS18 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RPS18